GRB14 Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, IF, E |
---|---|
Primary Accession | Q14449 |
Reactivity | Human |
Host | Rabbit |
Clonality | polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 60988 Da |
Gene ID | 2888 |
---|---|
Other Names | Growth factor receptor-bound protein 14, GRB14 adapter protein, GRB14 |
Target/Specificity | This GRB14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-48 amino acids from the human region of human GRB14. |
Dilution | IF~~1:25 WB~~1:1000 FC~~1:25 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GRB14 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GRB14 |
---|---|
Function | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. |
Cellular Location | Cytoplasm. Endosome membrane; Peripheral membrane protein. Note=Upon insulin stimulation, translocates to the plasma membrane. |
Tissue Location | Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
References
Daly R.J.,et al.J. Biol. Chem. 271:12502-12510(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Bereziat V.,et al.J. Biol. Chem. 277:4845-4852(2002).
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