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human IgG antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - human IgG antibody AP22392a
    All lanes : Anti-TSC2-S1798/S1799 at 1:2000 dilution Lane 1: LNCap whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 200kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - human IgG antibody AP22392a
    All lanes : Anti-TSC2-S1798/S1799 at 1:500 dilution Lane 1: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 200kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - human IgG antibody AP22392a
    Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(Cat#AP22392a). AP22392a was diluted at 1:1000 dilution. AmpSeeTM Detection Systems(ADR005) was used as the secondary antibody., followed by DAB staining.
    detail
  • SPECIFICATION
  • CITATIONS: 6
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P01857
Other Accession P01859, P01860, P01861
Reactivity Human
Host Polyclonal
Clonality Polyclonal
Calculated MW 43912 Da
Additional Information
Dilution WB~~1:500
IHC-P~~1:500-1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionshuman IgG antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGHG1 {ECO:0000303|PubMed:11340299, ECO:0000303|Ref.13}
Function Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins- secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus- infected cells.
Cellular Location [Isoform 1]: Secreted
Research Areas
Citations ( 0 )
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$ 365.00
$ 140.00
Cat# AP22392a
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