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AGL Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, E |
|---|---|
| Primary Accession | P35573 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 174764 Da |
| Gene ID | 178 |
|---|---|
| Other Names | Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1, 4-1, 4-glucantransferase, Amylo-alpha-1, 6-glucosidase, Amylo-1, 6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE |
| Target/Specificity | This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AGL. |
| Dilution | IF~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AGL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AGL |
|---|---|
| Synonyms | GDE |
| Function | Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation. |
| Cellular Location | Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus |
| Tissue Location | Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
References
Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002).
Shen, J., et al., Hum. Mutat. 9(1):37-40 (1997).
Bao, Y., et al., Genomics 38(2):155-165 (1996).
Shen, J., et al., J. Clin. Invest. 98(2):352-357 (1996).
Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992).
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