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Ribophorin 2 (RPN2) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, E |
|---|---|
| Primary Accession | P04844 |
| Other Accession | P25235, Q9GL01, Q3SZI6, NP_002942 |
| Reactivity | Human |
| Predicted | Bovine, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 69284 Da |
| Antigen Region | 16-46 aa |
| Gene ID | 6185 |
|---|---|
| Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, RIBIIR, Ribophorin II, RPN-II, Ribophorin-2, RPN2 |
| Target/Specificity | This Ribophorin 2 (RPN2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human Ribophorin 2 (RPN2). |
| Dilution | IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Ribophorin 2 (RPN2) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RPN2 (HGNC:10382) |
|---|---|
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Cellular Location | Endoplasmic reticulum {ECO:0000250|UniProtKB:F1PCT7}. Endoplasmic reticulum membrane; Multi- pass membrane protein |
| Tissue Location | Expressed in all tissues tested. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
RNP2 a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
References
Kelleher, D.J., et al., Mol. Cell 12(1):101-111 (2003). Fu, J., et al., J. Biol. Chem. 275(6):3984-3990 (2000). Loffler, C., et al., Hum. Genet. 87(2):221-222 (1991). Crimaudo, C., et al., EMBO J. 6(1):75-82 (1987). Stoffel, M., et al., Hum. Mol. Genet. 1 (8), 656 (1992).
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