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Ribophorin 2 (RPN2) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, E |
|---|---|
| Primary Accession | P04844 |
| Other Accession | P25235, Q9GL01, Q3SZI6, NP_002942 |
| Reactivity | Human |
| Predicted | Bovine, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 69284 Da |
| Antigen Region | 16-46 aa |
| Gene ID | 6185 |
|---|---|
| Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, RIBIIR, Ribophorin II, RPN-II, Ribophorin-2, RPN2 |
| Target/Specificity | This Ribophorin 2 (RPN2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human Ribophorin 2 (RPN2). |
| Dilution | IHC-P~~1:50~100 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Ribophorin 2 (RPN2) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RPN2 (HGNC:10382) |
|---|---|
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Cellular Location | Endoplasmic reticulum {ECO:0000250|UniProtKB:F1PCT7}. Endoplasmic reticulum membrane; Multi- pass membrane protein |
| Tissue Location | Expressed in all tissues tested. |
Provided below are standard protocols that you may find useful for product applications.
Background
RNP2 a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
References
Kelleher, D.J., et al., Mol. Cell 12(1):101-111 (2003). Fu, J., et al., J. Biol. Chem. 275(6):3984-3990 (2000). Loffler, C., et al., Hum. Genet. 87(2):221-222 (1991). Crimaudo, C., et al., EMBO J. 6(1):75-82 (1987). Stoffel, M., et al., Hum. Mol. Genet. 1 (8), 656 (1992).
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