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FDFT1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P37268 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 48115 Da |
| Antigen Region | 140-170 aa |
| Gene ID | 2222 |
|---|---|
| Other Names | Squalene synthase, SQS, SS, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase, FDFT1 |
| Target/Specificity | This FDFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-170 amino acids from the Central region of human FDFT1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | FDFT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FDFT1 |
|---|---|
| Function | Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q02769}; Multi-pass membrane protein |
| Tissue Location | Widely expressed.. |
Provided below are standard protocols that you may find useful for product applications.
Background
FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.
References
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Soltis, D.A., et al., Arch. Biochem. Biophys. 316(2):713-723 (1995).
Jiang, G., et al., J. Biol. Chem. 268(17):12818-12824 (1993).
Robinson, G.W., et al., Mol. Cell. Biol. 13(5):2706-2717 (1993).
Summers, C., et al., Gene 136 (1-2Che), 185-192 (1993).
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