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SAE1 (AOS1) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9UBE0 |
| Other Accession | Q6AXQ0, Q9R1T2, A2VE14 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 38450 Da |
| Antigen Region | 300-329 aa |
| Gene ID | 10055 |
|---|---|
| Other Names | SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A |
| Target/Specificity | This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1). |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SAE1 (AOS1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SAE1 |
|---|---|
| Synonyms | AOS1, SUA1, UBLE1A |
| Function | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Cellular Location | Nucleus. |
| Tissue Location | Expression level increases during S phase and drops in G2 phase (at protein level). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
References
Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999).
Gong, L., et al., FEBS Lett. 448(1):185-189 (1999).
Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
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