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DAX1 (NR0B1) Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DAX1 (NR0B1) Antibody (C-term) AP2708b
    Western blot analysis of NROB1 (arrow) using rabbit polyclonal NROB1 Antibody (Human C-term) (Cat.#AP2708b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NROB1 gene (Lane 2) (Origene Technologies).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P51843
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51718 Da
Antigen Region 304-333 aa
Additional Information
Gene ID 190
Other Names Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, NR0B1, AHC, DAX1
Target/Specificity This DAX1 (NR0B1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the C-terminal region of human DAX1 (NR0B1).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDAX1 (NR0B1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR0B1
Synonyms AHC, DAX1
Function Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus
Research Areas
Citations (0)
citation

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Background

NROB1contains a DNA-binding domain and acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in the NROB1 gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

References

Calliari,L.E., Genet. Mol. Res. 6 (2), 177-183 (2007)
Kinsey,M., Mol. Cancer Res. 4 (11), 851-859 (2006)

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$ 365.00
$ 140.00
Cat# AP2708b
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