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Prohibitin (PHB1) Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Prohibitin (PHB1) Antibody (N-term) AP2710a
    All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Prohibitin (PHB1) Antibody (N-term) AP2710a
    Immunohistochemical analysis of AP2710A on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - Prohibitin (PHB1) Antibody (N-term) AP2710a
    Immunohistochemical analysis of AP2710A on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - Prohibitin (PHB1) Antibody (N-term) AP2710a
    Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, E
Primary Accession P35232
Other Accession P84173, Q3T165
Reactivity Human, Mouse
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29804 Da
Antigen Region 92-121 aa
Additional Information
Gene ID 5245
Other Names Prohibitin, PHB
Target/Specificity This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1).
Dilution WB~~1:2000
IHC-P~~1:100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsProhibitin (PHB1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHB (HGNC:8912)
Function Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed:11302691, PubMed:20959514, PubMed:28017329, PubMed:31522117). Plays a role in adipose tissue and glucose Homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity).
Cellular Location Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane
Tissue Location Widely expressed in different tissues.
Research Areas
Citations (0)
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Background

Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.

References

Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008) White,J.J., Genomics 11 (1), 228-230 (1991)

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$ 360.00
$ 120.00
Cat# AP2710a
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