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Olfm1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Olfm1 Antibody (N-term) AP2719a
    All lanes: Anti-Olfm1 Antibody (N-term) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 55 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - Olfm1 Antibody (N-term) AP2719a
    Anti-Noelin-1(Olfm1) (N-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - Olfm1 Antibody (N-term) AP2719a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Olfm1 Antibody (N-term) (Cat.#AP2719a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q99784
Other Accession Q62609, O88998, Q9IAK4
Reactivity Human
Predicted Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55343 Da
Antigen Region 71-100 aa
Additional Information
Gene ID 10439
Other Names Noelin, Neuronal olfactomedin-related ER localized protein, Olfactomedin-1, OLFM1, NOE1, NOEL1
Target/Specificity This Olfm1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the N-terminal region of human Olfm1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOlfm1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OLFM1
Synonyms NOE1, NOEL1
Function Contributes to the regulation of axonal growth in the embryonic and adult central nervous system by inhibiting interactions between RTN4R and LINGO1. Inhibits RTN4R-mediated axon growth cone collapse (By similarity). May play an important role in regulating the production of neural crest cells by the neural tube (By similarity). May be required for normal responses to olfactory stimuli (By similarity).
Cellular Location Secreted {ECO:0000250|UniProtKB:O88998}. Synapse {ECO:0000250|UniProtKB:O88998}. Endoplasmic reticulum {ECO:0000250|UniProtKB:O88998}. Cell projection, axon {ECO:0000250|UniProtKB:O88998}. Perikaryon {ECO:0000250|UniProtKB:O88998}
Research Areas
Citations ( 0 )

Background

Olfm1 shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of this protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue.

References

Yokoyama M.,DNA Res. 3:311-320(1996).
Yu W., Genome Res. 7:353-358(1997).
Liu T.,J. Proteome Res. 4:2070-2080(2005).

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$ 182.50
$ 70.00
Cat# AP2719a
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