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GADD45A Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | P24522 |
| Other Accession | P48317, P48316, P24523, Q3ZBN6 |
| Reactivity | Human |
| Predicted | Bovine, Hamster, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 18336 Da |
| Antigen Region | 133-165 aa |
| Gene ID | 1647 |
|---|---|
| Other Names | Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45 |
| Target/Specificity | This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the C-terminal region of human GADD45A. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GADD45A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GADD45A |
|---|---|
| Synonyms | DDIT1, GADD45 |
| Function | In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. |
| Cellular Location | Nucleus. |
Provided below are standard protocols that you may find useful for product applications.
Background
GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
References
Li,L.S., J. Biol. Chem. 283 (31), 21394-21403 (2008)
Al-Romaih,K., Neoplasia 10 (5), 471-480 (2008)
Zhu,Q.S., Cancer Res. 68 (8), 2895-2903 (2008)
Kearsey,J.M., Oncogene 11 (9), 1675-1683 (1995)
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