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RFC2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | P35250 |
| Other Accession | Q641W4, Q05B83 |
| Reactivity | Human, Rat |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 39157 Da |
| Antigen Region | 17-46 aa |
| Gene ID | 5982 |
|---|---|
| Other Names | Replication factor C subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2 |
| Target/Specificity | This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human RFC2. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RFC2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RFC2 |
|---|---|
| Function | Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA (PubMed:9488738). This subunit binds ATP (By similarity). |
| Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome.
References
Tomida,J., J. Biol. Chem. 283 (14), 9071-9079 (2008) Gupte,R.S., Cell Cycle 4 (2), 323-329 (2005)
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