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AHSA1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AHSA1 Antibody (N-term) AP2892a
    Western blot analysis of AHSA1 Antibody (N-term) (Cat. #AP2892a) in HL-60, T47D cell line lysates (35ug/lane). AHSA1 (arrow) was detected using the purified Pab.
    detail
  • FC - AHSA1 Antibody (N-term) AP2892a
    AHSA1 Antibody (N-term) (Cat. #AP2892a) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession O95433
Other Accession Q8BK64
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38274 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 10598
Other Names Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38, AHSA1, C14orf3
Target/Specificity This AHSA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human AHSA1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAHSA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AHSA1
Synonyms C14orf3
Function Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co- chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155).
Cellular Location Cytoplasm, cytosol. Endoplasmic reticulum. Note=May transiently interact with the endoplasmic reticulum
Tissue Location Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
Research Areas
Citations (0)
citation

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Background

AHSA1 is a cochaperone that stimulates HSP90 ATPase activity. This protein may affect a step in the endoplasmic reticulum to Golgi trafficking.

References

Wang,X.,et. al., Cell 127 (4), 803-815 (2006)
Panaretou,B., et. al., Mol. Cell 10 (6), 1307-1318 (2002)

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$ 182.50
$ 70.00
Cat# AP2892a
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