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MDH2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MDH2 Antibody (Center) AP2909c
    MDH2 Antibody (Center) (Cat. #AP2909c) western blot analysis in A431,Hela and mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the MDH2 antibody detected the MDH2 protein (arrow).
    detail
  • IHC-P - MDH2 Antibody (Center) AP2909c
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - MDH2 Antibody (Center) AP2909c
    Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P40926
Other Accession P04636, P00346, P08249, Q4R568, O02640, Q32LG3, P22133
Reactivity Human, Mouse
Predicted Yeast, Bovine, C.Elegans, Monkey, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35503 Da
Antigen Region 86-115 aa
Additional Information
Gene ID 4191
Other Names Malate dehydrogenase, mitochondrial, MDH2
Target/Specificity This MDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the Central region of human MDH2.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMDH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MDH2
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P04636}
Research Areas
Citations ( 0 )

Background

MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

References

Rzem,R.,et.al., J. Inherit. Metab. Dis. 30 (5), 681-689 (2007)

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$ 365.00
$ 140.00
Cat# AP2909c
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