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GOT1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P17174 |
| Other Accession | Q4R5L1, P33097, P08906 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Horse, Monkey |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 46248 Da |
| Antigen Region | 5-33 aa |
| Gene ID | 2805 |
|---|---|
| Other Names | Aspartate aminotransferase, cytoplasmic, cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A, GOT1 |
| Target/Specificity | This GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human GOT1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GOT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GOT1 (HGNC:4432) |
|---|---|
| Function | Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2- aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456). |
| Cellular Location | Cytoplasm. |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
References
Panteghini,M. et.al,. Clin. Biochem. 23 (4), 311-319 (1990)
Doyle,J.M., et.al., Biochem. J. 270 (3), 651-657 (1990)
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$ 150.00
$ 385.00
Cat# AP2947A
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