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Phospho-CREB(S133) Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, IHC-P-Leica, E |
|---|---|
| Primary Accession | P16220 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35136 Da |
| Gene ID | 1385 |
|---|---|
| Other Names | Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1 |
| Target/Specificity | This CREB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB. |
| Dilution | WB~~1:500 IHC-P~~N/A IF~~1:50~200 IHC-P-Leica~~1:500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-CREB(S133) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CREB1 |
|---|---|
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083} |
Provided below are standard protocols that you may find useful for product applications.
Background
CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
References
Samten, B., et al., J. Immunol. 174(10):6357-6363 (2005).
Zhang, X., et al., Proc. Natl. Acad. Sci. U.S.A. 102(12):4459-4464 (2005).
Cho, K.N., et al., J. Biol. Chem. 280(8):6676-6681 (2005).
Bailey, J., et al., J. Mol. Endocrinol. 34(1):1-17 (2005).
Perry, C., et al., Neoplasia 6(3):279-286 (2004).
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