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Cleaved-CASP3 (Asp175) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Cleaved-CASP3 (Asp175) Antibody AP3725a
    Cleaved-CASP3 (Asp175)Antibody (Cat. #AP3725a) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-CASP3 (Asp175)Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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  • DB - Cleaved-CASP3 (Asp175) Antibody AP3725a
    Dot blot analysis of anti-Cleaved-CASP3 (Asp175) Antibody (Cat. #AP3725a) on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
    detail
  • SPECIFICATION
  • CITATIONS: 7
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, DB, E
Primary Accession P42574
Other Accession P55213, Q95ND5, P70677, Q08DY9
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31608 Da
Antigen Region 149-175 aa
Additional Information
Gene ID 836
Other Names Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32
Target/Specificity This Cleaved antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-175 amino acids from human Cleaved.
Dilution IHC-P~~1:50~100
DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCleaved-CASP3 (Asp175) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP3
Synonyms CPP32 {ECO:0000303|PubMed:7983002}
Function Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:7596430, PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:7596430, PubMed:18723680, PubMed:20566630, PubMed:23650375). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:7774019, PubMed:7596430, PubMed:10497198, PubMed:16374543). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35446120, PubMed:35338844). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486).
Cellular Location Cytoplasm.
Tissue Location Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Research Areas
Citations ( 0 )

Background

CASP3 encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

References

Mei, Y., et al. Mol. Cell 37(5):668-678(2010) Sohn, E.J., et al. Cancer Res. 70(3):1154-1163(2010) Karamitopoulou, E., et al. Pathology 42(1):37-42(2010)

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$ 365.00
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Cat# AP3725a
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