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Phospho-PBK/TOPK (Thr9) Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96KB5 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 36085 Da |
| Gene ID | 55872 |
|---|---|
| Other Names | Lymphokine-activated killer T-cell-originated protein kinase, 2.7.12.2, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK |
| Target/Specificity | This Phospho-PBK/TOPK (Thr9) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-35 amino acids from the human Phospho-PBK/TOPK. |
| Dilution | WB~~1:4000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-PBK/TOPK (Thr9) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PBK |
|---|---|
| Synonyms | TOPK |
| Function | Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin- induced DNA damage. |
| Tissue Location | Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
References
Abe Y.,et al.J. Biol. Chem. 275:21525-21531(2000).
Gaudet S.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:5167-5172(2000).
Zhao S.,et al.Int. J. Biochem. Cell Biol. 33:631-636(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
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