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A2ML1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| FC, WB, E |
|---|---|
| Primary Accession | A8K2U0 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 161107 Da |
| Antigen Region | 345-373 aa |
| Gene ID | 144568 |
|---|---|
| Other Names | Alpha-2-macroglobulin-like protein 1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9, A2ML1 {ECO:0000312|EMBL:AAI121321} |
| Target/Specificity | This A2ML1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-373 amino acids from the N-terminal region of human A2ML1. |
| Dilution | FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | A2ML1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | A2ML1 {ECO:0000312|EMBL:AAI12132.1} |
|---|---|
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases. |
| Cellular Location | Secreted. |
| Tissue Location | In the epidermis, expressed predominantly in the granular layer at the apical edge of keratinocytes (at protein level) Also detected in placenta, testis and thymus but not in epithelia of kidney, lung, small intestine or colon. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
A2ML1 is a new member of the alpha 2-macroglobulin protease inhibitor family, and shares most the characteristics of this family. It is the first family member detected in the epidermis and may play an important role during desquamation by inhibiting extracellular proteases.
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