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NAT8 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UHE5 |
| Other Accession | Q9UHF3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 25619 Da |
| Antigen Region | 110-138 aa |
| Gene ID | 9027 |
|---|---|
| Other Names | N-acetyltransferase 8, 231-, Acetyltransferase 2, ATase2, Camello-like protein 1, Cysteinyl-conjugate N-acetyltransferase, CCNAT, NAT8 (HGNC:18069) |
| Target/Specificity | This NAT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the Central region of human NAT8. |
| Dilution | IHC-P~~1:50~100 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NAT8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NAT8 (HGNC:18069) |
|---|---|
| Function | Endoplasmic reticulum (ER)-membrane-bound lysine N- acetyltransferase catalyzing the N6-acetylation of lysine residues in the lumen of the ER in various proteins, including PROM1 and BACE1, using acetyl-CoA as acetyl donor (PubMed:19011241, PubMed:22267734, PubMed:24556617, PubMed:31945187). Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617). May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241). N(6)-lysine acetylation in the ER maintains protein homeostasis and regulates reticulophagy (By similarity). Alternatively, acetylates the free alpha-amino group of cysteine S- conjugates to form mercapturic acids (PubMed:20392701). This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. |
| Cellular Location | Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein |
| Tissue Location | Preferentially expressed in liver and kidney. Also detected in brain (at protein level). |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
This protein, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.
References
Ko, M.H., et al. J. Biol. Chem. 284(4):2482-2492(2009) Juhanson, P., et al. BMC Med. Genet. 9, 25 (2008) Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
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$ 150.00
$ 385.00
Cat# AP4957c
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