|Application ||FC, IHC-P, WB, E|
|Other Accession||Q7TT45, Q99K70, Q9HB90|
|Calculated MW||45588 Da|
|Antigen Region||171-199 aa|
|Other Names||Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)|
|Target/Specificity||This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RRAGD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed:20381137, PubMed:24095279). Forms heterodimeric Rag complexes with RRAGA or RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form (PubMed:24095279). In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed:20381137, PubMed:24095279). This is a crucial step in the activation of the TOR signaling cascade by amino acids (PubMed:20381137, PubMed:24095279).|
|Cellular Location||Cytoplasm. Nucleus. Lysosome Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA (PubMed:11073942).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.
Olsen, J.V., et al. Cell 127(3):635-648(2006)
de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005)
Sekiguchi, T., et al. J. Biol. Chem. 279(9):8343-8350(2004)
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