RRAGD Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q9NQL2 |
Other Accession | Q7TT45, Q99K70, Q9HB90 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Calculated MW | 45588 Da |
Antigen Region | 171-199 aa |
Gene ID | 58528 |
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Other Names | Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903) |
Target/Specificity | This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RRAGD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RRAGD (HGNC:19903) |
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Function | Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed:20381137, PubMed:24095279). Forms heterodimeric Rag complexes with RRAGA or RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form (PubMed:24095279). In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed:20381137, PubMed:24095279). This is a crucial step in the activation of the TOR signaling cascade by amino acids (PubMed:20381137, PubMed:24095279). |
Cellular Location | Cytoplasm. Nucleus. Lysosome Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA (PubMed:11073942). |

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Provided below are standard protocols that you may find useful for product applications.
Background
RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.
References
Olsen, J.V., et al. Cell 127(3):635-648(2006)
de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005)
Sekiguchi, T., et al. J. Biol. Chem. 279(9):8343-8350(2004)

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