PLA2G4E Antibody
affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | Q3MJ16 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 98,57 KDa |
Antigen Region | 428-456 aa |
Gene ID | 123745 |
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Other Names | Cytosolic phospholipase A2 epsilon, cPLA2-epsilon, Phospholipase A2 group IVE, PLA2G4E |
Dilution | WB~~ 1:500-1:1000 IHC~~1:50-1:100 |
Format | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol. |
Storage Conditions | -20℃ |
Name | PLA2G4E (HGNC:24791) |
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Function | Calcium-dependent N-acyltransferase involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PubMed:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PubMed:29447909, PubMed:30517655). Has weak phospholipase A2 and lysophospholipase activities (By similarity). Regulates intracellular membrane trafficking that requires modulation of membrane curvature as it occurs by enrichment in lysophospholipids. Promotes tubule formation involved in clathrin-independent endocytotic trafficking and cargo recycling (By similarity). |
Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q50L42}. Early endosome membrane {ECO:0000250|UniProtKB:Q50L42}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q50L42}; Cytoplasmic side {ECO:0000250|UniProtKB:Q50L42}. Lysosome membrane {ECO:0000250|UniProtKB:Q50L42}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q50L42}; Cytoplasmic side {ECO:0000250|UniProtKB:Q50L42}. Cell membrane {ECO:0000250|UniProtKB:Q50L42}; Peripheral membrane protein; Cytoplasmic side {ECO:0000250|UniProtKB:Q50L42}. Note=Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates {ECO:0000250|UniProtKB:Q50L42} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position (By similarity).
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).
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