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AP1S1 Antibody (N-Term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | P61966 |
| Other Accession | P61967, Q1JQ98 |
| Reactivity | Human |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 18733 Da |
| Antigen Region | 5-34 aa |
| Gene ID | 1174 |
|---|---|
| Other Names | AP-1 complex subunit sigma-1A, Adaptor protein complex AP-1 subunit sigma-1A, Adaptor-related protein complex 1 subunit sigma-1A, Clathrin assembly protein complex 1 sigma-1A small chain, Clathrin coat assembly protein AP19, Golgi adaptor HA1/AP1 adaptin sigma-1A subunit, HA1 19 kDa subunit, Sigma 1a subunit of AP-1 clathrin, Sigma-adaptin 1A, Sigma1A-adaptin, AP1S1, AP19, CLAPS1 |
| Target/Specificity | This AP1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human AP1S1. |
| Dilution | FC~~1:10~50 IHC-P~~1:50~100 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AP1S1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AP1S1 |
|---|---|
| Synonyms | AP19, CLAPS1 |
| Function | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. |
| Cellular Location | Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex |
| Tissue Location | Widely expressed.. |
Provided below are standard protocols that you may find useful for product applications.
Background
AP1S1 is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle.
References
Montpetit, A., et al. PLoS Genet. 4 (12), E1000296 (2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Leong, W.F., et al. Cell. Microbiol. 8(4):565-580(2006)
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