PHF13 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q86YI8 |
Other Accession | Q8K2W6 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 33582 Da |
Antigen Region | 263-291 aa |
Gene ID | 148479 |
---|---|
Other Names | PHD finger protein 13, Survival time-associated PHD finger protein in ovarian cancer 1, SPOC1, PHF13 |
Target/Specificity | This PHF13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the C-terminal region of human PHF13. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PHF13 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PHF13 (HGNC:22983) |
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Function | Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response (PubMed:19638409). Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1 (PubMed:23034801). Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis (PubMed:19638409). Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation (PubMed:23034801). Essential for testicular stem-cell differentiation and sustained spermatogenesis (By similarity). |
Cellular Location | Nucleus. Nucleus, nucleoplasm. Note=Predominantly bound to chromatin, but a minor proportion is also detected in the nucleoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
PHF13 contains 1 PHD-type zinc finger. The function of PHF13 is currently unknown.
References
Kinkley, S., et al. J. Cell. Sci. 122 (PT 16), 2946-2956 (2009)
Mohrmann, G., et al. Int. J. Cancer 116(4):547-554(2005)
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