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PRM3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PRM3 Antibody  (C-term) AP5067b
    Western blot analysis of PRM3 Antibody (C-term) (Cat. #AP5067b) in Jurkat cell line lysates (35ug/lane).PRM3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - PRM3 Antibody  (C-term) AP5067b
    PRM3 Antibody (C-term) (Cat. #AP5067b) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRM3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - PRM3 Antibody  (C-term) AP5067b
    PRM3 Antibody (C-term) (Cat. #AP5067b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9NNZ6
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 11269 Da
Antigen Region 62-91 aa
Additional Information
Gene ID 58531
Other Names Protamine-3, Sperm protamine P3, PRM3
Target/Specificity This PRM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the C-terminal region of human PRM3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRM3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRM3
Function Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex (By similarity).
Cellular Location Nucleus. Chromosome.
Citations (0)
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Background

PRM3 Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex

References

Imken, L., et al. Mol. Hum. Reprod. 15(11):733-738(2009) Oliva, R. Hum. Reprod. Update 12(4):417-435(2006) Kramer, J.A., et al. Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 120(3):467-473(1998)

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$ 365.00
$ 140.00
Cat# AP5067b
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