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APAF-1 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, IHC-P, E |
|---|---|
| Primary Accession | O14727 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 37 KDa |
| Gene ID | 317 |
|---|---|
| Other Names | Apoptotic protease-activating factor 1, APAF-1, APAF1, KIAA0413 |
| Target/Specificity | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human APAF-1-ALT. The exact sequence is proprietary. |
| Dilution | WB~~1:1000 ICC~~N/A IHC-P~~N/A E~~N/A |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | APAF1 (HGNC:576) |
|---|---|
| Synonyms | KIAA0413 |
| Function | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
References
Zou H.,et al.Cell 90:405-413(1997).
Hahn C.,et al.Biochem. Biophys. Res. Commun. 261:746-749(1999).
Saleh A.,et al.J. Biol. Chem. 274:17941-17945(1999).
Hu Y.,et al.EMBO J. 18:3586-3595(1999).
Ogawa T.,et al.Biochem. Biophys. Res. Commun. 306:537-543(2003).
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