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Histone H4 (AcK12) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Histone H4 (AcK12) Antibody AP51256
    All lanes : Anti-Histone H4 (AcK12) Antibody at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P62805
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 11 KDa
Antigen Region 1 - 60 aa
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Target/Specificity KLH conjugated synthetic peptide derived from human Histone H4 (AcK12)
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name H4C1
Synonyms H4/A, H4FA, HIST1H4A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)
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Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

References

Sierra F.,et al.Nucleic Acids Res. 11:7069-7086(1983).
Pauli U.,et al.Science 236:1308-1311(1987).
Albig W.,et al.Genomics 10:940-948(1991).
Drabent B.,et al.DNA Cell Biol. 14:591-597(1995).
Albig W.,et al.Gene 184:141-148(1997).

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$ 350.00
Cat# AP51256
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