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RSPO2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IHC-P, E |
|---|---|
| Primary Accession | Q6UXX9 |
| Other Accession | Q8BFU0 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 28315 Da |
| Antigen Region | 190-218 aa |
| Gene ID | 340419 |
|---|---|
| Other Names | R-spondin-2, Roof plate-specific spondin-2, hRspo2, RSPO2 |
| Target/Specificity | This RSPO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-218 amino acids from the C-terminal region of human RSPO2. |
| Dilution | WB~~1:1000 FC~~1:10~50 IHC-P~~1:50~100 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RSPO2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RSPO2 |
|---|---|
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway (PubMed:21727895, PubMed:21909076, PubMed:22615920). During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form (PubMed:29769720). |
| Cellular Location | Secreted {ECO:0000250|UniProtKB:Q8BFU0}. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling.
References
Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)
Kazanskaya, O., et al. Dev. Cell 7(4):525-534(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
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