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E2F-1 Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, IP |
|---|---|
| Primary Accession | Q01094 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Calculated MW | 70 KDa |
| Gene ID | 1869 |
|---|---|
| Other Names | Dmel\CG6376 ; Dmel_CG6376 ; drosE2F1 ; E(Sev-CycE)3A ; E(var)3-93E ; E2-promoter binding facto ; E2F 1 ; E2F transcription factor 1 ; E2F-1 ; E2f-PA ; E2f-PB ; E2f-PC ; E2F1 ; E2f1 E2F transcription factor 1 ; E2F1_HUMAN ; Evar(3)164 ; KIAA4009 ; l(3)07172 ; l(3)j3B1 ; l(3)j3C2 ; l(3)rM729 ; mKIAA4009 ; OTTHUMP00000030661 ; PBR3 ; PRB binding protein E2F 1 ; PRB-binding protein E2F-1 ; RBAP 1 ; RBAP-1 ; RBAP1 ; RBBP-3 ; RBBP3 ; RBP 3 ; RBP3 ; Retinoblastoma-associated protein 1 ; Retinoblastoma-binding protein 3 ; Transcription factor E2F1. |
| Dilution | WB~~1:500 ICC~~1:100 IP~~1:500 |
| Format | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Name | E2F1 {ECO:0000303|PubMed:8964493, ECO:0000312|HGNC:HGNC:3113} |
|---|---|
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed:10675335, PubMed:12717439, PubMed:17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed:10675335, PubMed:12717439, PubMed:17704056). It can mediate both cell proliferation and TP53/p53- dependent apoptosis (PubMed:8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Directly activates transcription of PEG10 (PubMed:17050006, PubMed:18625225, PubMed:28992046). Positively regulates transcription of RRP1B (PubMed:20040599). |
| Cellular Location | Nucleus |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
References
Helin K.,et al.Cell 70:337-350(1992).
Kaelin W.G. Jr.,et al.Cell 70:351-364(1992).
Shan B.,et al.Mol. Cell. Biol. 12:5620-5631(1992).
Neuman E.,et al.Gene 173:163-169(1996).
Deloukas P.,et al.Nature 414:865-871(2001).
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