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Rabbit IgG (5B2) Mouse mAb

     
  • WB - Rabbit IgG (5B2) Mouse mAb AP53497
    Western blot detection of MEK-2 in THP-1,Jurkat,Raji,3T3 and Hela cell lysates using 310001(MEK-2 rabbit pAb,1:1000 diluted),then incubated with AP53497(Rabbit IgG mAb,1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.
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  • WB - Rabbit IgG (5B2) Mouse mAb AP53497
    Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using Mouse Anti-Rabbit IgG (Heavy-Chain Specific) mAb (1:1000 diluted).
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  • IP - Rabbit IgG (5B2) Mouse mAb AP53497
    Immunoprecipitation analysis of CHO-K1 cell lysates transfected with HA-tagged NGAL using HA-Tag Rabbit pAb (390001,1:100 diluted) (lane 2). Lane 1 contains rabbit IgG IP control lysate. All lanes are probed with HA-Tag Rabbit pAb (390001,1:1000 diluted) as the primary antibody. Panel A incubated with secondary antibodies Anti-Rabbit whole IgG, HRP-linked Antibody for detecting.Panel B incubated with Anti-Rabbit IgG (Heavy-Chain Specific) mAb(1:50000 diluted) and the Anti-mouse whole IgG, HRP-linked Antibody was used for detecting.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Host Mouse
Clonality Monoclonal Antibody
Additional Information
Other Names Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS.
Dilution WB~~1:1000
Research Areas
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$ 385.00
Cat# AP53497
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