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Anti-HOXA11/D11 Antibody

     
  • WB - Anti-HOXA11/D11 Antibody AP53680
    Western blot analysis of HOXA11/D11 expression in HEK293T (A), HeLa (B), K562 (C) whole cell lysates.
    detail
  • IH - Anti-HOXA11/D11 Antibody AP53680
    Immunohistochemical analysis of HOXA11/D11 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-HOXA11/D11 Antibody AP53680
    Immunofluorescent analysis of HOXA11/D11 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IH
Primary Accession P31270
Other Accession P31277
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 34486 Da
Additional Information
Gene ID 3207
Other Names HOXA11; HOX1I; Homeobox protein Hox-A11; Homeobox protein Hox-1I; HOXD11; HOX4F; Homeobox protein Hox-D11; Homeobox protein Hox-4F
Target/Specificity Recognizes endogenous levels of HOXA11/D11 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HOXA11
Synonyms HOX1I
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Citations (0)
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Background

Rabbit polyclonal antibody to HOXA11/D11

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$ 350.00
Cat# AP53680
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