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Anti-Cyclin A1/2 Antibody

     
  • WB - Anti-Cyclin A1/2 Antibody AP53716
    Western blot analysis of Cyclin A1/2 expression in HEK293T (A), NIH3T3 (B), rat brain (C), SW626 (D), SKOV3 (E), A2780 (F) whole cell lysates.
    detail
  • IH - Anti-Cyclin A1/2 Antibody AP53716
    Immunohistochemical analysis of Cyclin A1/2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Cyclin A1/2 Antibody AP53716
    Immunofluorescent analysis of Cyclin A1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession P78396
Other Accession P20248
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 52358 Da
Additional Information
Gene ID 8900
Other Names CCNA1; Cyclin-A1; CCNA2; CCN1; CCNA; Cyclin-A2; Cyclin-A
Target/Specificity Recognizes endogenous levels of Cyclin A1/2 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CCNA1
Function May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
Cellular Location Nucleus.
Tissue Location Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to Cyclin A1/2

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$ 350.00
Cat# AP53716
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