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Anti-NT5C1B Antibody

     
  • WB - Anti-NT5C1B Antibody AP53793
    Western blot analysis of NT5C1B expression in BV2 (A), MCF7 (B), H1792 (C) whole cell lysates.
    detail
  • IH - Anti-NT5C1B Antibody AP53793
    Immunohistochemical analysis of NT5C1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-NT5C1B Antibody AP53793
    Immunofluorescent analysis of NT5C1B staining in EAhy926 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession Q96P26
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 68804 Da
Additional Information
Gene ID 100526794;93034
Other Names AIRP; Cytosolic 5'-nucleotidase 1B; cN1B; Autoimmune infertility-related protein; Cytosolic 5'-nucleotidase IB; cN-IB
Target/Specificity Recognizes endogenous levels of NT5C1B protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NT5C1B
Synonyms AIRP
Function Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, AMP is the major substrate.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9BXI3}.
Tissue Location Highly expressed in testis, placenta and pancreas. Detected at lower levels in heart, kidney, liver and lung
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Background

Rabbit polyclonal antibody to NT5C1B

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$ 350.00
Cat# AP53793
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