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>   home   >   Products   >   Primary Antibodies   >   Anti-MED21 Antibody   

Anti-MED21 Antibody

     
  • WB - Anti-MED21 Antibody AP54024
    Western blot analysis of MED21 expression in PC12 (A), CT26 (B), HEK293T (C), SGC7901 (D), HCT116 (E) whole cell lysates.
    detail
  • IH - Anti-MED21 Antibody AP54024
    Immunohistochemical analysis of MED21 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q13503
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 15564 Da
Additional Information
Gene ID 9412
Other Names SRB7; SURB7; Mediator of RNA polymerase II transcription subunit 21; Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7
Target/Specificity Recognizes endogenous levels of MED21 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name MED21
Synonyms SRB7, SURB7
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Cellular Location Nucleus.
Citations (0)
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Background

Rabbit polyclonal antibody to MED21

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$ 350.00
Cat# AP54024
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