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Anti-PKI beta Antibody

     
  • WB - Anti-PKI beta Antibody AP54025
    Western blot analysis of PKI beta expression in BV2 (A), PC12 (B), U87MG (C) whole cell lysates.
    detail
  • IH - Anti-PKI beta Antibody AP54025
    Immunohistochemical analysis of PKI beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-PKI beta Antibody AP54025
    Immunofluorescent analysis of PKI beta staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession Q9C010
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 8468 Da
Additional Information
Gene ID 5570
Other Names PRKACN2; cAMP-dependent protein kinase inhibitor beta; PKI-beta
Target/Specificity Recognizes endogenous levels of PKI beta protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PKIB
Synonyms PRKACN2
Function Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
Research Areas
Citations (0)
citation

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Background

Rabbit polyclonal antibody to PKI beta

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$ 350.00
Cat# AP54025
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