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Anti-ECRG4 Antibody

     
  • WB - Anti-ECRG4 Antibody AP54026
    Western blot analysis of ECRG4 expression in U87MG (A), HEK293T (B), A549 (C), PC12 (D), CT26 (E) whole cell lysates.
    detail
  • IH - Anti-ECRG4 Antibody AP54026
    Immunohistochemical analysis of ECRG4 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-ECRG4 Antibody AP54026
    Immunofluorescent analysis of ECRG4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IH
Primary Accession Q9H1Z8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 17183 Da
Additional Information
Gene ID 84417
Other Names ECRG4; Augurin; Esophageal cancer-related gene 4 protein
Target/Specificity Recognizes endogenous levels of ECRG4 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ECRG4 (HGNC:24642)
Synonyms C2orf40
Function Probable hormone that may attenuate cell proliferation and induce senescence of oligodendrocyte and neural precursor cells in the central nervous system (By similarity). ECRG4-induced senescence is characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1 and CCND3 proteasomal degradation (By similarity).
Cellular Location Secreted. Cytoplasm. Apical cell membrane
Tissue Location Expressed in the brain, with expression in the epithelial cell layer of the choroid plexus (at protein level)
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to ECRG4

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$ 350.00
Cat# AP54026
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