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Anti-SPTBN5 Antibody

     
  • WB - Anti-SPTBN5 Antibody AP54052
    Western blot analysis of SPTBN5 expression in HEK293T (A), SGC7901 (B), Panc1 (C) whole cell lysates.
    detail
  • IH - Anti-SPTBN5 Antibody AP54052
    Immunohistochemical analysis of SPTBN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-SPTBN5 Antibody AP54052
    Immunocytochemistry analysis of SPTBN5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IC, IH
Primary Accession Q9NRC6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 416750 Da
Additional Information
Gene ID 51332
Other Names BSPECV; HUBSPECV; HUSPECV; Spectrin beta chain non-erythrocytic 5; Beta-V spectrin
Target/Specificity Recognizes endogenous levels of SPTBN5 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/100 - 1/500
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SPTBN5
Synonyms BSPECV, HUBSPECV, HUSPECV
Cellular Location Cytoplasm, cytoskeleton. Note=Detected prominently in the outer segments of photoreceptor rods and cones and in the basolateral membrane and cytosol of gastric epithelial cells
Tissue Location Expressed at very low levels in many tissues, with strongest expression in cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder, and heart
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to SPTBN5

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$ 350.00
Cat# AP54052
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