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Anti-ELOA3 Antibody

     
  • WB - Anti-ELOA3 Antibody AP54069
    Western blot analysis of ELOA3 expression in SKOVCAR3 (A), Panc1 (B) whole cell lysates.
    detail
  • IF - Anti-ELOA3 Antibody AP54069
    Immunofluorescent analysis of ELOA3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q8IYF1
Other Accession Q8NG57, Q3SY89, A6NLF2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 83921 Da
Additional Information
Gene ID 51224
Other Names TCEB3B; TCEB3L; RNA polymerase II transcription factor SIII subunit A2; Elongin-A2; EloA2; Transcription elongation factor B polypeptide 3B; TCEB3C; TCEB3L2; RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcription elongation factor B polypeptide 3C
Target/Specificity Recognizes endogenous levels of ELOA3 protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ELOA2 (HGNC:30771)
Synonyms TCEB3B, TCEB3L
Function SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A2 is transcriptionally active but its transcription activity is not enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
Cellular Location Nucleus.
Tissue Location Specifically expressed in testis.
Citations (0)
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Background

Rabbit polyclonal antibody to ELOA3

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$ 350.00
Cat# AP54069
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