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Anti-MXD4 Antibody

     
  • WB - Anti-MXD4 Antibody AP54092
    Western blot analysis of MXD4 expression in NIH3T3 (A), PMVEC (B), Hela (C), A375 (D) whole cell lysates.
    detail
  • IH - Anti-MXD4 Antibody AP54092
    Immunohistochemical analysis of MXD4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q14582
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 23528 Da
Additional Information
Gene ID 10608
Other Names BHLHC12; MAD4; Max dimerization protein 4; Max dimerizer 4; Class C basic helix-loop-helix protein 12; bHLHc12; Max-associated protein 4; Max-interacting transcriptional repressor MAD4
Target/Specificity Recognizes endogenous levels of MXD4 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name MXD4
Synonyms BHLHC12, MAD4
Function Transcriptional repressor. Binds with MAX to form a sequence- specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
Citations (0)
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Background

Rabbit polyclonal antibody to MXD4

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$ 350.00
Cat# AP54092
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