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Anti-OCT1 Antibody

     
  • WB - Anti-OCT1 Antibody AP54135
    Western blot analysis of 42278 expression in Hela (A), Jurkat (B), HepG2 (C) whole cell lysates.
    detail
  • IH - Anti-OCT1 Antibody AP54135
    Immunohistochemical analysis of 42278 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P14859
Other Accession P09086
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 76472 Da
Additional Information
Gene ID 5451
Other Names OCT1; OTF1; POU domain class 2 transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
Target/Specificity Recognizes endogenous levels of OCT1 protein.
Dilution WB~~1/500 - 1/2000
IH~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name POU2F1
Synonyms OCT1, OTF1
Function Transcription factor that binds to the octamer motif (5'- ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.
Cellular Location Nucleus.
Tissue Location Ubiquitous. Isoform 2 is lymphocyte-specific.
Citations (0)
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Background

Mouse monoclonal antibody to OCT1

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$ 350.00
Cat# AP54135
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