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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   ECM1 Polyclonal Antibody   

ECM1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - ECM1 Polyclonal Antibody AP54209
    Tissue/cell: mouse endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ECM1 Polyclonal Antibody, Unconjugated(bs-0776R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - ECM1 Polyclonal Antibody AP54209
    Sample:
    MDA-MB-231(Mouse) Cell Lysate at 30 ug
    MCF-7(Human) Cell Lysate at 30 ug
    Primary: Anti-ECM1 (bs-0776R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 59 kD
    Observed band size: 65 kD
    detail
  • WB - ECM1 Polyclonal Antibody AP54209
    Sample:
    HepG2(Human) Cell Lysate at 30 ug
    Primary: Anti-ECM1 (bs-0776R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 59 kD
    Observed band size: 64 kD
    detail
  • WB - ECM1 Polyclonal Antibody AP54209
    Sample:
    MCF-7(Human) Cell Lysate at 30 ug
    Primary: Anti-ECM1 (bs-0776R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 59 kD
    Observed band size: 64 kD
    detail
  • WB - ECM1 Polyclonal Antibody AP54209
    Sample:
    Skin(Mouse) Lysate at 40 ug
    Primary: Anti-ECM1 (bs-0776R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 59 kD
    Observed band size: 64 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q61508
Reactivity Rat, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 62832 Da
Additional Information
Gene ID 13601
Other Names Extracellular matrix protein 1, Secretory component p85, Ecm1
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name Ecm1
Function Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Expressed in the surrounding connective tissues of developing long bones, but not in the cartilage. The long isoform is expressed in a number of tissues including liver, heart and lungs. The short isoform is expressed in skin and cartilage-containing tissues such as tail and front paw. No expression is found in brain
Research Areas
Citations (0)
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$ 350.00
Cat# AP54209
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