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Angiopoietin 4 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Angiopoietin 4 Polyclonal Antibody AP54315
    Tissue/cell: mouse tumor tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ANGPT3/ANG3/ANGPT4/ANG4 Polyclonal Antibody, Unconjugated(bs-1087R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - Angiopoietin 4 Polyclonal Antibody AP54315
    Sample:
    Lung (Mouse) Lysate at 30 ug
    Lung (Rat) Lysate at 30 ug
    Primary: Anti- Anti-ANGPT3/ANG3/ANGPT4/ANG4 (bs-1087R) at 1/400 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
    Predicted band size : 57kD
    Observed band size : 57kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession Q9Y264
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 56849 Da
Additional Information
Gene ID 51378
Other Names Angiopoietin-4, ANG-4, Angiopoietin-3, ANG-3, ANGPT4, ANG3, ANG4
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ANGPT4
Synonyms ANG3, ANG4
Function Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
Cellular Location Secreted.
Tissue Location Highly expressed in the lung with much lower levels found in other tissues
Research Areas
Citations (0)
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$ 350.00
Cat# AP54315
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