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ILVBL Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ILVBL Polyclonal Antibody AP54341
    Sample:
    Hcclm3(Human) Cell Lysate at 30 ug
    MCF-7(Human) Cell Lysate at 30 ug
    A431(Human) Cell Lysate at 30 ug
    Primary: Anti- ILVBL (bs-11014R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 68 kD
    Observed band size: 68 kD
    detail
  • WB - ILVBL Polyclonal Antibody AP54341
    Sample: HepG2 Cell (Human) Lysate at 30 ug
    Primary: Anti-ILVBL (bs-11014R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 68kD
    Observed band size: 68kD
    detail
  • FC - ILVBL Polyclonal Antibody AP54341
    Blank control: HepG2.
    Primary Antibody (green line): Rabbit Anti-ILVBL antibody (bs-11014R)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1 µg /test.
    Protocol
    The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession A1L0T0
Reactivity Rat, Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 67868 Da
Additional Information
Gene ID 10994
Other Names 2-hydroxyacyl-CoA lyase 2, 4.1.2.-, Acetolactate synthase-like protein, IlvB-like protein, ILVBL (HGNC:6041)
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=3ug/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ILVBL (HGNC:6041)
Function Endoplasmic reticulum 2-OH acyl-CoA lyase involved in the cleavage (C1 removal) reaction in the fatty acid alpha-oxydation in a thiamine pyrophosphate (TPP)-dependent manner. Involved in the phytosphingosine degradation pathway.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Expressed in all tissues tested, with highest expression in heart, pancreas and placenta
Research Areas
Citations (0)
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$ 350.00
Cat# AP54341
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