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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Monoamine Oxidase B Polyclonal Antibody   

Monoamine Oxidase B Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Monoamine Oxidase B Polyclonal Antibody AP54448
    Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Monoamine Oxidase B) Polyclonal Antibody, Unconjugated (bs-11297R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - Monoamine Oxidase B Polyclonal Antibody AP54448
    Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Monoamine Oxidase B) Polyclonal Antibody, Unconjugated (bs-11297R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - Monoamine Oxidase B Polyclonal Antibody AP54448
    Sample: Raji Cell (Human) Lysate at 40 ug
    Primary: Anti-Monoamine Oxidase B (bs-11297R) at 1/300 dilution
    Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
    Predicted band size: 59 kD
    Observed band size: 59 kD
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession P27338
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 58763 Da
Additional Information
Gene ID 4129
Other Names Amine oxidase [flavin-containing] B, 1.4.3.4, Monoamine oxidase type B, MAO-B, MAOB
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name MAOB (HGNC:6834)
Function Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed:11134050, PubMed:8665924, PubMed:8316221, PubMed:11049757, PubMed:20493079). Preferentially degrades benzylamine and phenylethylamine (PubMed:11134050, PubMed:8665924, PubMed:8316221, PubMed:11049757, PubMed:20493079).
Cellular Location Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side
Research Areas
Citations (0)
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$ 350.00
Cat# AP54448
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