RDH16 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
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Primary Accession | O75452 |
Other Accession | NP_003699.3 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 35673 Da |
Antigen Region | 3-31 aa |
Gene ID | 8608 |
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Other Names | Retinol dehydrogenase 16, 11--, Microsomal NAD(+)-dependent retinol dehydrogenase 4, RoDH-4, Sterol/retinol dehydrogenase, RDH16, RODH4 |
Target/Specificity | This RDH16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-31 amino acids from the N-terminal region of human RDH16. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RDH16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RDH16 (HGNC:29674) |
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Function | Oxidoreductase with a preference for NAD. Oxidizes all-trans- retinol, 9-cis-retinol, 11-cis-retinol and 13-cis-retinol to the corresponding aldehydes (PubMed:10329026, PubMed:12534290, PubMed:9677409). Has higher activity towards CRBP-bound retinol than with free retinol (PubMed:12534290). Oxidizes also 3-alpha- hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction (PubMed:10329026, PubMed:9677409, PubMed:29541409). |
Cellular Location | Microsome membrane. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O54909}; Single- pass membrane protein |
Tissue Location | Highly expressed in adult liver (at protein level) (PubMed:9677409). Detected in endometrium, liver and foreskin (PubMed:10329026, PubMed:11967490). Detected in the spineous layers of adult skin, and at lower levels in basal and granular skin layers (PubMed:10329026). Detected in fetal liver and lung |
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Provided below are standard protocols that you may find useful for product applications.
Background
Retinol dehydrogenase 16 (RDH16) is an oxidoreductase with a preference for NAD. It oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. RDH16 has higher activity towards CRBP-bound retinol than with free retinol. It oxidizes 3-alpha-hydroxysteroids and also oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. RDH16 can also catalyze the reverse reaction.
References
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009) :
Lapshina, E.A., et al. Biochemistry 42(3):776-784(2003)
Cain, J.M., et al. Am. J. Obstet. Gynecol. 186(4):675-683(2002)
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