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UBXD2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UBXD2 Polyclonal Antibody AP54592
    Sample:
    Heart (Mouse) Lysate at 40 ug
    Cerebrum (Rat) Lysate at 40 ug
    Primary: Anti-UBXD2   (bs-11675R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 57 kD
    Observed band size: 57 kD
    detail
  • IHC-P - UBXD2 Polyclonal Antibody AP54592
    Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (bs-11675R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    detail
  • IHC-P - UBXD2 Polyclonal Antibody AP54592
    Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (bs-11675R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q92575
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 56778 Da
Additional Information
Gene ID 23190
Other Names UBX domain-containing protein 4, Erasin, UBX domain-containing protein 2, UBXN4, KIAA0242, UBXD2, UBXDC1
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name UBXN4
Synonyms KIAA0242, UBXD2, UBXDC1
Function Involved in endoplasmic reticulum-associated protein degradation (ERAD). Acts as a platform to recruit both UBQLN1 and VCP to the ER during ERAD (PubMed:19822669).
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus envelope. Note=Both the N- and the C-terminus face the cytosol. Also found in the nucleus envelope contiguous to the ER
Tissue Location Expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas Accumulates in Alzheimer disease-afflicted brains (at protein level)
Research Areas
Citations (0)
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$ 350.00
Cat# AP54592
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