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P2Y9 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - P2Y9 Polyclonal Antibody AP54719
    Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (bs-12074R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • WB - P2Y9 Polyclonal Antibody AP54719
    Sample: k562 (human)cell Lysate at 40 ug
    Primary: Anti- P2Y9 (bs-12074R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 48 kD
    detail
  • WB - P2Y9 Polyclonal Antibody AP54719
    Sample: Brain (mouse) Lysate at 40 ug
    Primary: Anti- P2Y9 (bs-12074R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 48 kD
    detail
  • WB - P2Y9 Polyclonal Antibody AP54719
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Ovary (Mouse) Lysate at 40 ug
    Primary: Anti-P2Y9 (bs-12074R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 57 kD
    detail
  • WB - P2Y9 Polyclonal Antibody AP54719
    Sample:
    HL-60(Human) Cell Lysate at 30 ug
    K562(Human) Cell Lysate at 30 ug
    Primary: Anti-P2Y9 (bs-12074R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 42 kD
    Observed band size: 57 kD
    detail
  • WB - P2Y9 Polyclonal Antibody AP54719
    Sample:
    HL-60 Cell (Human) Lysate at 30 ug
    Primary: Anti-P2Y9 (bs-12074R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q8BLG2
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 41899 Da
Additional Information
Gene ID 78134
Other Names Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, G-protein coupled receptor 23, P2Y purinoceptor 9, P2Y9, Purinergic receptor 9, Lpar4, Gpr23, Lpa4, P2y9
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name Lpar4
Synonyms Gpr23, Lpa4, P2y9
Function Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1- oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1- alkenyl-LPA (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas
Citations (0)
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$ 350.00
Cat# AP54719
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