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ZNF342 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - ZNF342 Polyclonal Antibody AP54771
    Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-ZNF342 Polyclonal Antibody, Unconjugated(bs-12212R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - ZNF342 Polyclonal Antibody AP54771
    Sample: Kidney (Mouse) Lysate at 40 ug
    Primary: Anti-ZNF342 (bs-12212R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q8WUU4
Reactivity Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 51 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from Human ZNF342/ZNF296
Epitope Specificity 231-350/475
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nuclear
SIMILARITY Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the Krüppel C2H2-type zinc-finger protein family, ZNF342 (zinc finger protein 342), also known as Zinc finger protein 296, is a 475 amino acid nuclear protein that contains six C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation.
Additional Information
Gene ID 162979
Other Names Zinc finger protein 296, ZFP296, Zinc finger protein 342, ZNF296, ZNF342
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ZNF296
Synonyms ZNF342
Function May be a transcriptional corepressor with KLF4.
Cellular Location Nucleus {ECO:0000250|UniProtKB:E9Q6W4}.
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$ 385.00
Cat# AP54771
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