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TUG Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TUG Polyclonal Antibody AP54836
    Sample:
    Heart (Mouse) Lysate at 40 ug
    Primary: Anti-TUG  (bs-12355R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 58 kD
    detail
  • IHC-P - TUG Polyclonal Antibody AP54836
    Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUG) Polyclonal Antibody, Unconjugated (bs-12355R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - TUG Polyclonal Antibody AP54836
    Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUG) Polyclonal Antibody, Unconjugated (bs-12355R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q9BZE9
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 60183 Da
Additional Information
Gene ID 79058
Other Names Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, ASPSCR1, ASPL, RCC17, TUG, UBXD9, UBXN9
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ASPSCR1
Synonyms ASPL, RCC17, TUG, UBXD9, UBXN9
Function Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT.
Cellular Location Endomembrane system; Peripheral membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Peripheral membrane protein. Cytoplasm Nucleus
Tissue Location Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
Research Areas
Citations (0)
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$ 350.00
Cat# AP54836
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