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PFDN3 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PFDN3 Polyclonal Antibody AP54909
    Sample:
    HepG2(Human) Cell Lysate at 40 ug
    Raji(Human) Cell Lysate at 40 ug
    Primary: Anti-PFDN3 (bs-10196R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 23 kD
    Observed band size: 23 kD
    detail
  • IHC-P - PFDN3 Polyclonal Antibody AP54909
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PFDN3) Polyclonal Antibody, Unconjugated (bs-12628R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession P61758
Host Rabbit
Clonality Polyclonal
Calculated MW 22626 Da
Additional Information
Gene ID 7411
Other Names Prefoldin subunit 3, HIBBJ46, von Hippel-Lindau-binding protein 1, VBP-1, VHL-binding protein 1, VBP1, PFDN3
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name VBP1
Synonyms PFDN3
Function Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Cellular Location Cytoplasm. Nucleus. Note=In complex with VHL can translocate to the nucleus
Tissue Location Ubiquitous.
Research Areas
Citations (0)
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$ 350.00
Cat# AP54909
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Availability: 2 weeks
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