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USP17L24 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - USP17L24 Antibody (C-term) AP5491b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-251 MG cells labeling USP17L24 with AP5491b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on U-251 MG cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
  • WB - USP17L24 Antibody (C-term) AP5491b
    All lanes : Anti-USP17L24 Antibody (C-term) at 1:1000 dilution Lane 1: U-87 MG whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - USP17L24 Antibody (C-term) AP5491b
    USP17L24 Antibody (C-term) (Cat.#AP5491b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the USP17L24 antibody detected the USP17L24 protein (arrow).
  • WB - USP17L24 Antibody (C-term) AP5491b
    Western blot analysis of lysate from MAD-MB-231 cell line, using USP17L24 Antibody (C-term)(Cat. #AP5491b). AP5491b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • IHC-P-Leica - USP17L24 Antibody (C-term) AP5491b
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP5491b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • FC - USP17L24 Antibody (C-term) AP5491b
    USP17L24 Antibody (C-term) (Cat. #AP5491b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • detail
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, IF, WB, FC, E
Primary Accession Q0WX57
Other Accession C9JPN9, D6RA61, D6R901, D6RJB6, D6RCP7, D6R9N7, D6RBQ6, C9J2P7, C9JLJ4, C9JVI0, A8MUK1, XP_001130410.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 59711 Da
Antigen Region 496-524 aa
Additional Information
Gene ID 728369;728373;728379;728393;728400;728405;728419
Other Names Ubiquitin carboxyl-terminal hydrolase 17-like protein 24, Deubiquitinating enzyme 17, Ubiquitin thioesterase 17, Ubiquitin-specific-processing protease 17, USP17L24
Target/Specificity This USP17L24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the C-terminal region of human USP17L24.
Dilution IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP17L24 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP17L24
Function Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apoptosis, cell migration, and the cellular response to viral infection.
Cellular Location Nucleus, nucleolus. Endoplasmic reticulum
Tissue Location Expressed in heart, brain, liver and skeletal muscle.
Research Areas
Citations ( 0 )


USP17 recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins (By similarity).

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$ 365.00
$ 140.00
Cat# AP5491b
Availability: In Stock
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